PortaCellTec (PCT) provides a broad spectrum of hepatic/renal uptake transporter assays (e.g. OATs, OCTs, OATPs) to investigate the inhibitor and substrate characteristics of your drug. As recommended by FDA and EMA we perform in vitro transporter studies with validated drug transporters stably-transfected in HEK293 cells.
Inhibition studies: Interaction analysis of a test substance with specific transporters by means of a reference substrate.
Direct uptake: Transporter uptake assay for a labeled test substance.
Transport kinetics: Determination of time- and concentration-dependent uptake of a test substance
Determination of IC50 value: Concentration dependent inhibition of a reference substrate by a test substance.
Determination of the inhibition character: Dixon Plot analysis to determine competitive or non-competitive inhibition.
Determination of affinity: Concentration dependent uptake of a labeled test substance to determine the affinity of a transporter for this substance. We also detect the maximum transport rate (Vmax) from this data simultaneously.
In May 2012, PD Dr. Yohannes Hagos (CEO of PortaCellTec biosciences) was awarded by the business region Goettingen with the “Gute Gründe(r) ” price. Wolfgang Meyer (Mayor of Goettingen), Klaus Hoffmann (CEO of GWG) and Detlev Barth (CEO of WRG) handed him the award for the implementation of an innovative and viable business idea. Over 3 years PortaCellTec successfully provides drug transporter assays.